Steven Graves

 

Steven W. Graves
Associate Professor
Associate Director, Center for Biomedical Engineering
Centennial Engineering Center - Room 2041

505 277.2043
graves@unm.edu



Education:

  • Postdoctoral Research Associate The National Flow Cytometry Resource, Los Alamos National Laboratory, 1999-2001
  • Ph.D. The Pensylvania State University Biochemistry, Microbiology, Molecular Biology, 1998
    Thesis "Cloning, Expression, Purification, and Complete Kinetic Characterization of the Large Sub-Unit of Human Mitochondrial DNA Polymerase"
  • B.A. University of Colorado, Boulder Biochemistry and Molecular Biology (Double Major) 1991


Research

  • Biomolecular Assemblies
  • Protease Mechanisms
  • Point of Care Medical Devices
  • Flow Cytometry

 

Professional Experience

  • 2008 - Present: Associate Professor, Department of Chemical & Nuclear Engineering and Department of Chemistry • University of New Mexico, Albuquerque, NM.
  • 2008 - Present: Associate Director, Center for Biomedical Engineering, School of Engineering • University of New Mexico, Albuquerque, NM.
  • 2002 - Present: Project Leader, The National Flow Cytometry Resource, Los Alamos National Laboratory, Los Alamos, NM.
  • 2007 - 2008: Team Leader, Optical Spectroscopy and Instrumentation, Biosciences Division, Los Alamos National Laboratory, Los Alamos, NM.
  • 2001-08: Technical Staff Member, Biosciences Division, Los Alamos National Laboratory, Los Alamos, NM.
  • 1999-2001: Postdoctoral Research Associate, The National Flow Cytometry Resource, Los Alamos National Laboratory, Los Alamos, NM.

Teaching

  • ChNE 406/506 Biomedical Engineering Seminar • Fall 2008.
  • ChNE 499/599 Special Topics: Biological Macromolecular Engineering • Spring 2009.


Ongoing Research Support:

  • National Institute of Health • 08/05-07/10
    Low cost portable flow cytometry - NIH RR020064 R21/R33
    This is a grant to develop a low cost portable flow cytometer for use in resource poor settings for the diagnosis of critical diseases such as HIV. In this work we are developing new technologies for fluidics, optics, and data acquisition that will transform a flow cytometer from a costly bulky clinical instrument into a true point of care device.
  • National Institute of Health • 07/07-06/12
    The National Flow Cytometry Resource - NIH RR01315-25
    Next Generation Large Particle Sorting
    Overall Project Center Goal: To provide a national resource in flow cytometry with research, collaborations, service training and dissemination activities. Project Lead on - Next Generation Optically Activated Large Particle Sorting. With few exceptions, cell sorters rely on droplet based sorting of particles. While this works well for smaller particles, because of fluid dynamic effects the size of particles that can be sorted is limited to ~100 µm with rates of less than 1000 events/sec. Using a combination of synchronous particle delivery, droplet on demand, parallel flow cells, low cost excitation and emission detection components and miniaturized data acquisition we will develop high-thoughput large particle sorting systems. Our target is to be able to sort particles up to 1 mm at rates of more than 10,000 events/sec. This will have large impacts on combinatorial library screening and multi-cellular organism sorting.

Selected Publications:

  • Watson, D. A., Brown, L. O., Graham, D. A., Naivar, M. A., Graves, S. W., Doorn, S. K., Nolan J. P., A flow Cytometer for the Measurement of Raman Spectra, Cytometry Part A. 2008 Feb;73(2):119-28.
  • Goddard G.R., Sanders C., Martin J.C., Kaduchak G., Graves S.W. Analytical performance of an ultrasonic particle focusing flow cytometer, Anal. Chem., 2007. Nov 15;79(22):8740-6.
  • Deshpande A, Hammon RJ, Sanders CK, Graves SW. (2006) Quantitative analysis of the effect of cell type and cellular differentiation on protective antigen binding to human target cells. FEBS Lett. Jul 24;580(17):4172-5.
  • Saunders MJ, Kim H, Woods TA, Nolan JP, Sklar LA, Edwards BS, Graves SW (2006).Microsphere-based protease assays and screening application for lethal factor and factor Xa. Cytometry A. May;69(5):342-52.
  • Graves, S. W., Nolan, J. P., Jett, J. H., Martin, J. C. and Sklar, L.A. (2002) Nozzle design parameters and their effects on rapid sample delivery in flow cytometry. Cytometry 47(2):127-37.
  • Johnson, A.A., Tsai, Y., Graves S.W. and Johnson, K.A. (2000) Human mitochondrial DNA polymerase holoenzyme: Reconstitution and characterization. Biochemistry, 39(7), 1702-1708.
  • Graves S.W., Johnson A.A. and Johnson K.A. (1998). Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. Biochemistry, 37(17) 6050-6058.

Total Publications in Career:

  • 24 publications, 1 patents awarded, 2 patents pending.